Anti-Human BMP-4 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
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Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Preparation | Produced in mice using highly pure (>98%) recombinant human BMP-4 as the immunizing antigen. This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography. |
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WesternBlot | To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions. |
Sandwich | In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 2000 pg/ml of recombinant human BMP-4 when used in conjunction with compatible secondary reagents. |
Immunohistochemistry | This antibody stained HepG2 cells. The primary antibody was incubated at 3.0 µg/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm. |
Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP-4 (5.0 ng/ml), a concentration of 0.4-0.6 μg/ml of this antibody is required. |
Formulation | A sterile filtered antibody solution was lyophilized from PBS. |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
Storage | -20°C |
Precautions | Anti-Human BMP-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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