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Anti-Cyclin D2 Antibody (Monoclonal, DCS-3)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC |
|---|---|
| Primary Accession | Q04827 |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Reactivity | Human, Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for Cyclin D2, cyclin D2 (CCND2) detection. Tested with WB, ICC in Human;mouse. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 64033 |
|---|---|
| Other Names | G1/S-specific cyclin-D2, Vin-1 proto-oncogene, Ccnd2, Vin-1 |
| Calculated MW | 32826 MW KDa |
| Application Details | Immunocytochemistry , 1 µg/ml, Human, mouse, - Western blot, 2-4 µg/ml, Human, mouse |
| Subcellular Localization | Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. . |
| Protein Name | G1/S-specific cyclin-D2 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | DCS-3 |
| Immunogen | Recombinant human cyclin D2 protein. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the cyclin family. Cyclin D subfamily. |
| Name | Ccnd2 |
|---|---|
| Synonyms | Vin-1 |
| Function | Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:P30279}. Cytoplasm {ECO:0000250|UniProtKB:P30279}. Nucleus membrane {ECO:0000250|UniProtKB:P30279}. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members {ECO:0000250|UniProtKB:P30279} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Inaba et al.(1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2(CCND2) gene ia assigned to 12p13.
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