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Anti-CACYBP Antibody

     
  • WB - Anti-CACYBP Antibody ABO11068
    Anti-CACYBP antibody, ABO11068, Western blottingAll lanes: Anti CACYBP (ABO11068) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Rat Spleen Tissue Lysate at 50ugLane 4: SMMC Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: SW620 Whole Cell Lysate at 40ugLane 7: 293T Whole Cell Lysate at 40ugPredicted bind size: 26KDObserved bind size: 26KD
    detail
  • ICC - Anti-CACYBP Antibody ABO11068
    Anti-CACYBP antibody, ABO11068, ICCICC: A549 Cell
    detail
  • IHC - Anti-CACYBP Antibody ABO11068
    Anti-CACYBP antibody, ABO11068,IHC(P)IHC(P): Rat Brain Tissue
    detail
  • IHC - Anti-CACYBP Antibody ABO11068
    Anti-CACYBP antibody, ABO11068,IHC(P)IHC(P): Human Liver Cancer Tissue
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC
Primary Accession Q9HB71
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Calcyclin-binding protein(CACYBP) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 27101
Other Names Calcyclin-binding protein, CacyBP, hCacyBP, S100A6-binding protein, Siah-interacting protein, CACYBP, S100A6BP, SIP
Calculated MW 26210 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat

Immunocytochemistry , 0.5-1 µg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Nucleus . Cytoplasm . Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
Protein Name Calcyclin-binding protein(CacyBP/hCacyBP)
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human CACYBP (160-175aa NTRWDYLTQVEKECKE), identical to the related rat and mouse sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Contains 1 CS domain.
Protein Information
Name CACYBP
Synonyms S100A6BP, SIP
Function May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
Cellular Location Nucleus. Cytoplasm. Note=Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated
Research Areas
Citations (0)
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Background

CACYBP(Calcyclin-binding protein), also called SIP, is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short(SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.

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$ 280.00
Cat# ABO11068
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