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Anti-VDAC/Porin Antibody

     
  • WB - Anti-VDAC/Porin Antibody ABO11086
    Anti-VDAC/Porin antibody, ABO11086, Western blottingAll lanes: Anti VDAC/Porin (ABO11086) at 0.5ug/mlLane 1: Rat Skeletal Muscle Tissue Lysate at 50ugLane 2: Rat Heart Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: A431 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: SMMC Whole Cell Lysate at 40ugLane 8: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 31KDObserved bind size: 31KD
    detail
  • IHC - Anti-VDAC/Porin Antibody ABO11086
    Anti-VDAC/Porin antibody, ABO11086, IHC(P)IHC(P): Rat Cardiac Muscle Tissue
    detail
  • IHC - Anti-VDAC/Porin Antibody ABO11086
    Anti-VDAC/Porin antibody, ABO11086, IHC(P)IHC(P): Rat Skeletal Muscle Tissue
    detail
  • ICC - Anti-VDAC/Porin Antibody ABO11086
    Anti-VDAC/Porin antibody, ABO11086, ICCICC: NIH3T3 Cell
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, ICC
Primary Accession P21796
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Voltage-dependent anion-selective channel protein 1(VDAC1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 7416
Other Names Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC1, VDAC
Calculated MW 30773 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Mitochondrion outer membrane . Cell membrane .
Tissue Specificity Heart, liver and skeletal muscle.
Protein Name Voltage-dependent anion-selective channel protein 1(VDAC-1/hVDAC1)
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human VDAC(163-178aa RVTQSNFAVGYKTDEF), identical to the related rat and mouse sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the eukaryotic mitochondrial porin family.
Protein Information
Name VDAC1
Synonyms VDAC
Function Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterol cholesterol (PubMed:31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). May mediate ATP export from cells (PubMed:30061676).
Cellular Location Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Membrane raft; Multi-pass membrane protein
Tissue Location Expressed in erythrocytes (at protein level) (PubMed:27641616). Expressed in heart, liver and skeletal muscle (PubMed:8420959).
Research Areas
Citations (0)
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Background

The voltage-dependent anion channel(VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is though to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. At low transmembrane voltage, VDAC is open for anions such as phosphate, chloride, and adenine nucleotides. At higher transmembrane voltage, VDAC functions as a selective channel for cations and uncharged molecules. These features make VDAC likely to play a role in mitochondrial energy metabolism. Huizing et al. studied by Northern and Western blot analyses the human tissue distribution of mitochondrial transmembrane metabolite carriers. They found that VDAC1 mRNA has a ubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas the VDAC2 isoform appears to be expressed only in the heart.

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$ 280.00
Cat# ABO11086
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