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Anti-HSPB2 Antibody
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- BACKGROUND
Application 
| WB, IHC-F, ICC |
|---|---|
| Primary Accession | Q16082 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Heat shock protein beta-2(HSPB2) detection. Tested with WB, IHC-F, ICC in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 3316 |
|---|---|
| Other Names | Heat shock protein beta-2, HspB2, DMPK-binding protein, MKBP, HSPB2 |
| Calculated MW | 20233 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Human, Mouse Immunocytochemistry , 0.5-1 µg/ml, Human, Mouse, Rat |
| Subcellular Localization | Cytoplasm . Nucleus . Localizes to nuclear foci. |
| Tissue Specificity | Expressed preferentially in skeletal muscle and heart but not in the lens. |
| Protein Name | Heat shock protein beta-2(HspB2) |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human HSPB2(102-118aa RHPQRLDRHGFVSREFC), identical to the related rat and mouse sequences. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the small heat shock protein (HSP20) family. |
| Name | HSPB2 |
|---|---|
| Function | May regulate the kinase DMPK. |
| Cellular Location | Cytoplasm. Nucleus. Note=Localizes to nuclear foci |
| Tissue Location | Expressed preferentially in skeletal muscle and heart but not in the lens |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein(HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al.(1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al.(1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYAB gene is highly expressed in all 3 tissues. The 2 related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.
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