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Anti-Mitochondrial Pyruvate Dehydrogenase Kinase 1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | Q15118 |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial(PDK1) detection. Tested with WB, IHC-P in Human;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 5163 |
|---|---|
| Other Names | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, 2.7.11.2, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1, PDK1, PDHK1 |
| Calculated MW | 49244 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, By Heat Western blot, 0.1-0.5 µg/ml, Human, Rat |
| Subcellular Localization | Mitochondrion matrix . |
| Tissue Specificity | Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas. . |
| Protein Name | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1(408-421aa WKHYNTNHEADDWC), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the PDK/BCKDK protein kinase family. |
| Name | PDK1 |
|---|---|
| Synonyms | PDHK1 |
| Function | Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. |
| Cellular Location | Mitochondrion matrix |
| Tissue Location | Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas |
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Provided below are standard protocols that you may find useful for product applications.
Background
PDK1(Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.
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