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Anti-Hepsin Antibody
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- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P05981 |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Serine protease hepsin(HPN) detection. Tested with WB in Human;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 3249 |
|---|---|
| Other Names | Serine protease hepsin, 3.4.21.106, Transmembrane protease serine 1, Serine protease hepsin non-catalytic chain, Serine protease hepsin catalytic chain, HPN, TMPRSS1 |
| Calculated MW | 45011 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat |
| Subcellular Localization | Membrane; Single-pass type II membrane protein. |
| Tissue Specificity | Present in most tissues, with the highest level in liver. |
| Protein Name | Serine protease hepsin |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Hepsin(392-410aa KVSDFREWIFQAIKTHSEA), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the peptidase S1 family. |
| Name | HPN |
|---|---|
| Synonyms | TMPRSS1 |
| Function | Serine protease that cleaves extracellular substrates, and contributes to the proteolytic processing of growth factors, such as HGF and MST1/HGFL (PubMed:21875933, PubMed:15839837). Plays a role in cell growth and maintenance of cell morphology (PubMed:8346233, PubMed:21875933). Plays a role in the proteolytic processing of ACE2 (PubMed:24227843). Mediates the proteolytic cleavage of urinary UMOD that is required for UMOD polymerization (PubMed:26673890). |
| Cellular Location | Cell membrane; Single-pass type II membrane protein Apical cell membrane; Single-pass type II membrane protein |
| Tissue Location | Detected in liver and kidney. |
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Provided below are standard protocols that you may find useful for product applications.
Background
HPN(Hepsin), also known as TMPRSS1, is an enzyme that in humans is encoded by the HPN gene. Tsuji et al.(1991) mapped the hepsin gene to 19q11-q13.2. Using cDNA microarrays, Dhanasekaran et al.(2001) examined gene expression profiles of more than 50 normal and neoplastic prostate specimens and 3 common prostate cancer cell lines. Signature expression profiles of normal adjacent prostate, benign prostatic hypertrophy, localized prostate cancer, and metastatic, hormone-refractory prostate cancer were determined. In 3 different human cell lines, Guipponi et al.(2008) found that TMPRSS1 was stably anchored in the endoplasmic reticulum.
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