c-FLIP Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF |
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Primary Accession | O15519 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 55344 Da |
Gene ID | 8837 |
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Application & Usage | Western blot analysis (0.5-4 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually. The immunoaffinity-purified antibody detects 58 kDa FLIPL on SDS-PAGE immunoblots, in samples from human, mouse and rat origins. The antibody recognizes only the FLIPL form. |
Other Names | CFLAR , I-FLICE, CASP8AP1, Casper, Usurpin, c-FLIPS , FLAME , CASH , c-FLIPL, CLARP, c-FLIPR, c-FLIP , MRIT , FLAME1 |
Target/Specificity | c-FLIP |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.2 mg/ml) affinity purified rabbit anti-FLIP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | c-FLIP Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CFLAR |
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Synonyms | CASH, CASP8AP1, CLARP, MRIT |
Function | Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. |
Tissue Location | Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle |
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Provided below are standard protocols that you may find useful for product applications.
Background
c-FLIP was cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH AND CLARP. c-FLIP contains two death effector domains and has sequence homology to caspase-8. c-FLIP interacts with adapter protein FADD and caspaser-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Two splice variants of c-FLIP have been identified and termed FLIPl and FLIPs
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