EDEM2 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | Q9BV94 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 64753 Da |
Gene ID | 55741 |
---|---|
Positive Control | Western Blot: Rat kidney lysate |
Application & Usage | Western blot: 1:200 |
Other Names | ER degradation-enhancing alpha-mannosidase-like protein 2 |
Target/Specificity | EDEM2 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | EDEM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | EDEM2 |
---|---|
Synonyms | C20orf31, C20orf49 |
Function | Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner (PubMed:15537790, PubMed:25092655). May initiate ERAD by promoting the first mannose trimming step of ERAD substrates, from Man9GlcNAc2 to Man8GlcNAc2 (PubMed:25092655). Seems to recognize and bind to exposed hydrophobic regions in target proteins (By similarity). |
Cellular Location | Endoplasmic reticulum lumen |
Tissue Location | Expressed ubiquitously in all tissues tested with slightly higher levels detected in small intestine and peripheral blood leukocytes and weakest levels in brain and skeletal muscle |
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Provided below are standard protocols that you may find useful for product applications.
Background
Proteins expressed in the endoplasmic reticulum (ER) are tightly regulated by a variety of quality control mechanisms. Terminally misfolded proteins in the ER are retrotranslocated to the cytoplasm and degraded by proteasomes through a mechanism known as ER-associated degradation (ERAD). EDEM2 (ER degradation-enhancing alpha-mannosidase-like 2) is a 578 amino acid secreted protein that, in conjunction with other EDEM proteins (namely EDEM and EDEM3), is involved in the ERAD pathway of protein degradation. EDEM2, a member of the glycosyl hydrolase 47 family, contains a mannosidase homology domain, an N-terminal cleavable signal sequence and a C-terminal extension that is required for both ER retention and the proper function of EDEM2. Human EDEM2 shares 93% sequence identity with its mouse homolog, suggesting a conserved role between species. Two isoforms of EDEM2 exist due to alternative splicing events.
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