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Goat Anti-Calponin 2 / CNN2 Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-Calponin 2 / CNN2 Antibody AF1180a
    AF1180a (0.03 µg/ml) staining of HepG2 lysates (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q99439
Other Accession NP_958434, 1265
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 33697 Da
Additional Information
Gene ID 1265
Other Names Calponin-2, Calponin H2, smooth muscle, Neutral calponin, CNN2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Calponin 2 / CNN2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CNN2
Function Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue Location Heart and smooth muscle.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Two transcript variants encoding different isoforms have been found for this gene.

References

h2-Calponin is regulated by mechanical tension and modifies the function of actin cytoskeleton. Hossain MM, et al. J Biol Chem, 2005 Dec 23. PMID 16236705.
Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.
Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Gevaert K, et al. Nat Biotechnol, 2003 May. PMID 12665801.
Developmentally regulated expression of calponin isoforms and the effect of h2-calponin on cell proliferation. Hossain MM, et al. Am J Physiol Cell Physiol, 2003 Jan. PMID 12388067.
The DNA sequence of human chromosome 21. Hattori M, et al. Nature, 2000 May 18. PMID 10830953.

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$ 341.00
Cat# AF1180a
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