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CYP7B1 Antibody (C-Term)

Peptide-affinity purified goat antibody

     
  • IHC - CYP7B1 Antibody (C-Term) AF2380a
    AF2380a (4 µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC, E
Primary Accession O75881
Other Accession NP_004811.1, 9420
Predicted Human, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 58256 Da
Additional Information
Gene ID 9420
Other Names 25-hydroxycholesterol 7-alpha-hydroxylase, 1.14.13.100, Cytochrome P450 7B1, Oxysterol 7-alpha-hydroxylase, CYP7B1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP7B1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP7B1 {ECO:0000303|PubMed:24491228, ECO:0000312|HGNC:HGNC:2652}
Function A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids (PubMed:10588945, PubMed:24491228). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:10588945, PubMed:24491228). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position (PubMed:10588945, PubMed:24491228). Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3- hydroxy steroid 7-alpha hydroxylase (PubMed:24491228). Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene- 3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids (PubMed:9802883, PubMed:10588945). Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen- specific humoral immune response (By similarity). 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning (PubMed:24491228). Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives (PubMed:24491228).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi- pass membrane protein
Tissue Location Widely expressed. Expressed in brain, testis, ovary, prostate, liver, colon, kidney, small intestine, thymus and spleen.
Research Areas
Citations (0)
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References

Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1. Wu Z, Martin KO, Javitt NB, Chiang JY. J Lipid Res. 1999 Dec;40(12):2195-203. PMID: 10588945

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$ 341.00
Cat# AF2380a
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