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GCC185 Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • IHC - GCC185 Antibody (internal region) AF2615a
    AF2615a (3.8 µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC, E
Primary Accession Q8IWJ2
Other Accession NP_055450.1, NP_852118.1, 9648
Reactivity Human
Predicted Rat
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 195910 Da
Additional Information
Gene ID 9648
Other Names GRIP and coiled-coil domain-containing protein 2, 185 kDa Golgi coiled-coil protein, GCC185, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, Ran-binding protein 2-like 4, RanBP2L4, Renal carcinoma antigen NY-REN-53, GCC2, KIAA0336, RANBP2L4
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGCC185 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GCC2
Synonyms KIAA0336, RANBP2L4
Function Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi- associated microtubules through recruitment of CLASP1 and CLASP2.
Cellular Location Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein
Tissue Location Ubiquitous..
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).

References

Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network. Efimov A, Kharitonov A, Efimova N, Loncarek J, Miller PM, Andreyeva N, Gleeson P, Galjart N, Maia AR, McLeod IX, Yates JR 3rd, Maiato H, Khodjakov A, Akhmanova A, Kaverina I. Dev Cell. 2007 Jun;12(6):917-30. PMID: 17543864

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$ 341.00
Cat# AF2615a
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