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CCL21 Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - CCL21 Antibody (internal region) AF3191a
    AF3191a (0.1 µg/ml) staining of Human Lymph Node lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • WB - CCL21 Antibody (internal region) AF3191a
    EB10008 (0.1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, Pep-ELISA
Primary Accession O00585
Other Accession NP_002980.1, 6366
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 14646 Da
Additional Information
Gene ID 6366
Other Names C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, Secondary lymphoid-tissue chemokine, SLC, Small-inducible cytokine A21, CCL21, SCYA21
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCL21 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCL21
Synonyms SCYA21
Function Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Cellular Location Secreted.
Tissue Location Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen
Research Areas
Citations (0)
citation

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Background

This antibody may cross-react with mouse CCL21 (GeneID: 18829) and rat CCL21 (GeneID: 298006).

References

Dendritic cell transfected with secondary lymphoid-tissue chemokine and/or interleukin-2 gene-enhanced cytotoxicity of T-lymphocyte in human bladder tumor cell S in vitro. Li YG, Wang ZP, Tian JQ, Tian BQ, Rodrigues R, Shang PF, Zhang T, Cancer investigation 2009 Nov 27 (9): 909-17. PMID: 19832038

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$ 341.00
Cat# AF3191a
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