|Calculated MW||28055 Da|
|Homology||Unlikely to recognize mouse or human samples.|
|Other Names||Aquaporin-8, AQP-8, Aqp8|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)REIKGKETNMADSYH, corresponding to amino acid residues 14-28 of rat AQP-8 (Accession number P56405). Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Aquaporin 8 (AQP-8) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12. The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water and include besides AQP-8, AQP-1, AQP-2, AQP4 and AQP-5, and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.2 AQP-8 is expressed along the gastrointestinal tract including small intestine, colon, pancreas and liver and also in other organs such as testis, lung and kidney. The physiological function of AQP-8 is not entirely clear but studies suggest that it has an important role in colonic water adsorption and hepatocyte bile formation.3,4 Interestingly, AQP-8 expression is markedly altered in gastrointestinal disorders such as ulcerative colitis and inflammatory bowel disease.5
References 1. Zahn, A. et al. (2007) World J. Gastroenterol. 13, 1687. 2. Laforenza, U. et al. (2005) J. Nutr. 135, 2329. 3. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. 4. Ma, T. and Verkman, A.S. (1999) J. Physiol. 517, 317. 5. Koyama, Y. et al. (1997) J Biol Chem 272, 30329
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.