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Vesicular GABA Transporter Antibody

Affinity purified polyclonal antibody

  • WB - Vesicular GABA Transporter Antibody AG1231-025
    Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human U-87 MG glyoblastoma lysates (lanes 3 and 6): 1-3. Anti-Vesicular GABA Transporter antibody (#AG1231), (1:200).
    4-6. Anti-Vesicular GABA Transporter antibody, preincubated with the control peptide antigen.
  • IHC - Vesicular GABA Transporter Antibody AG1231-025
    Expression of Vesicular GABA Transporter in rat cerebellum Immunohistochemical staining of rat frozen cerebellum sections using Anti-Vesicular GABA Transporter antibody (#AG1231), (1:100). VGAT staining is shown in red and dapi (blue) was used as a general cellular marker. VGAT appears in the cerebellar pinceau (arrows) and in the soma of Purkinje cells (triangles).
  • ICC - Vesicular GABA Transporter Antibody AG1231-025
    Colocalization of AMPA Receptor 1 (GluR1) and Vesicular GABA Transporter in human U-87 MG cells Immunocytochemical staining of human glioblastoma U-87 MG. Extracellular staining of live intact cells with Anti-AMPA Receptor 1 (GluR1) (extracellular) antibody (#AGP-009), (1:25), followed by goat anti-guinea pig-AlexaFluor-488 secondary antibody (green). Cells were subsequently fixed, permeabilized and labeled with Anti-Vesicular GABA Transporter antibody (#AG1231), (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). Representative merged images of the double labeled cells are shown in A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O35458
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 57407 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 83612
Other Names Vesicular inhibitory amino acid transporter, GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, rGVAT, Slc32a1, Vgat, Viaat
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (Accession O35458). Cytoplasmic, N-terminus
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 1 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody
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The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one family member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)1. VGAT is responsible for the uptake of GABA and Glycine in exchange of protons, and their release from nerve terminals1. The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail2. In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown3. VGAT is expressed on synaptic vesicles of GABAergic and glycynergic neurons4-6. The transporter is also detected in the pituitary gland and in the pancreas7,8. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat vesicular GABA transporter. Anti-Vesicular GABA Transporter antibody (#AG1231) can be used in western blot analysis. It has been designed to recognize VGAT from human, mouse and rat samples.


References 1. Gasnier, B. (2004) Pflugers Arch. 447, 756. 2. McIntire, S. et al. (1997) Nature 389, 870. 3. Bedet, C. et al. (2000) J. Neurochem. 75, 1654. 4. Chaudry, F. et al. (1998) J. Neurosci. 18, 9733. 5. Dumoulin, A. et al. (1999) J. Cell Sci. 112, 811. 6. Takamori, S. et al. (2000) J. Neurosci. 20, 4904. 7. Mayerhofer, A. et al. (2001) FASEB J. 15, 1089. 8. Chessler, S. et al. (2002) Diabetes 51, 1763.

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$ 247.00
$ 367.00
$ 475.00
Cat# AG1231-025
Availability: 2-3 weeks
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