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TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide

Mouse Monoclonal Antibody [Clone SPM357 ]

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC
Primary Accession P01135
Other Accession 7039, 170009
Reactivity Human, Mouse, Rat, Cow
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1, kappa
Clone Names SPM357
Calculated MW ~6kDa
Additional Information
Gene ID 7039
Other Names Protransforming growth factor alpha, Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1, TGFA
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TGFA
Function TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Cellular Location [Transforming growth factor alpha]: Secreted, extracellular space
Tissue Location Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines
Research Areas
Citations (0)
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Background

This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGFĪ � (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

References

Kobrin MS et. al. J Bio Chem, 1986, 261:14414-9. | Kudlow JE et. al. Endocrinology, 1987, 121(4):1577-9. | Kobrin MS et. al. Endocrinology, 1987, 121(4):1412-6. | Kudlow JE et. al. J Bio Chem, 1989, 264(7):3880-3 |

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$ 219.00
$ 499.00
Cat# AH10761-20
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