AMACR Antibody (clone 2A10F3)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q9UHK6 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 2A10F3 |
Calculated MW | 42kDa |
Dilution | ELISA (1:10000), IF (1:200-1:1000), IHC-P (1:200), WB (1:500-1:2000) , |
Gene ID | 23600 |
---|---|
Other Names | Alpha-methylacyl-CoA racemase, 5.1.99.4, 2-methylacyl-CoA racemase, AMACR |
Target/Specificity | Purified truncated recombinant AMACR-His fusion protein expressed in E. Coli strain BL21 (DE3) |
Precautions | AMACR Antibody (clone 2A10F3) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | AMACR |
---|---|
Function | Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:7649182, PubMed:10655068, PubMed:11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2- (4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:7649182, PubMed:10655068, PubMed:11060359). |
Cellular Location | Peroxisome. Mitochondrion |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
References
Amery L.,et al.J. Lipid Res. 41:1752-1759(2000).
Ferdinandusse S.,et al.Nat. Genet. 24:188-191(2000).
Mubiru J.N.,et al.Prostate 65:117-123(2005).
Albers C.,et al.Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
Ouyang B.,et al.Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
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