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ABHD5 Antibody (clone 1F3)

Mouse Monoclonal Antibody

     
  • WB - ABHD5 Antibody (clone 1F3) ALS14230
    ABHD5 monoclonal antibody, clone 1F3. Western blot of ABHD5 expression in human liver.
    detail
  • WB - ABHD5 Antibody (clone 1F3) ALS14230
    ABHD5 monoclonal antibody, clone 1F3. Western blot of ABHD5 expression in Jurkat.
    detail
  • IHC - ABHD5 Antibody (clone 1F3) ALS14230
    Anti-ABHD5 / CGI-58 antibody IHC of human skin.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q8WTS1
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1F3
Calculated MW 39kDa
Dilution IHC-P (5 µg/ml), WB (1:500-1:1000),
Additional Information
Gene ID 51099
Other Names 1-acylglycerol-3-phosphate O-acyltransferase ABHD5, 2.3.1.51, Abhydrolase domain-containing protein 5, Lipid droplet-binding protein CGI-58, ABHD5, NCIE2
Target/Specificity Human Abhd5
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsABHD5 Antibody (clone 1F3) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABHD5 (HGNC:21396)
Synonyms NCIE2
Function Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid (PubMed:18606822). Functions preferentially with 1-oleoyl- lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1- stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis (PubMed:18606822). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed:16679289). Involved in keratinocyte differentiation (PubMed:18832586). Regulates lipid droplet fusion (By similarity).
Cellular Location Cytoplasm. Lipid droplet {ECO:0000250|UniProtKB:Q9DBL9}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9DBL9}. Note=Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA (By similarity).
Tissue Location Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).
Research Areas
Citations (0)
citation

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Background

Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.

References

Lefevre C.,et al.Am. J. Hum. Genet. 69:1002-1012(2001).
Lai C.-H.,et al.Genome Res. 10:703-713(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 533.50
Cat# ALS14230
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