PPM1A / PP2CA Antibody (clone 4E11)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | P35813 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 4E11 |
Calculated MW | 42kDa |
Dilution | IHC-P (5 µg/ml), WB (1:500-1:1000), |
Gene ID | 5494 |
---|---|
Other Names | Protein phosphatase 1A, 3.1.3.16, Protein phosphatase 2C isoform alpha, PP2C-alpha, Protein phosphatase IA, PPM1A, PPPM1A |
Target/Specificity | Human PPM1A |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
Precautions | PPM1A / PP2CA Antibody (clone 4E11) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PPM1A |
---|---|
Synonyms | PPPM1A |
Function | Enzyme with a broad specificity. Negatively regulates TGF- beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha- mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB. |
Cellular Location | Nucleus. Cytoplasm, cytosol. Membrane; Lipid- anchor Note=Weakly associates at the membrane and N-myristoylation mediates the membrane localization. {ECO:0000250|UniProtKB:P49443} |
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Background
Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
References
Mann D.J.,et al.Biochim. Biophys. Acta 1130:100-104(1992).
Takekawa M.,et al.EMBO J. 17:4744-4752(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Goshima N.,et al.Nat. Methods 5:1011-1017(2008).
Heilig R.,et al.Nature 421:601-607(2003).
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