IPO5 / RANBP5 Antibody (clone 1C4)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | O00410 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 1C4 |
Calculated MW | 124kDa |
Dilution | IHC-P (5 µg/ml) |
Gene ID | 3843 |
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Other Names | Importin-5, Imp5, Importin subunit beta-3, Karyopherin beta-3, Ran-binding protein 5, RanBP5, IPO5, KPNB3, RANBP5 |
Target/Specificity | Human Importin 5 |
Reconstitution & Storage | Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles. |
Precautions | IPO5 / RANBP5 Antibody (clone 1C4) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | IPO5 |
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Synonyms | KPNB3, RANBP5 |
Function | Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed:9687515, PubMed:11682607). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation (By similarity). In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. |
Cellular Location | Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Nucleus; nuclear rim. Found particularly in the nuclear rim and nucleolus |
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Background
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
References
Yaseen N.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:4451-4456(1997).
Deane R.,et al.Mol. Cell. Biol. 17:5087-5096(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dunham A.,et al.Nature 428:522-528(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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