ACOT13 / THEM2 Antibody (aa100-150)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P |
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Primary Accession | Q9NPJ3 |
Reactivity | Human, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 15kDa |
Dilution | IHC-P (10 µg/ml), WB (0.05-0.1 µg/ml), |
Gene ID | 55856 |
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Other Names | Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13, 3.1.2.-, Thioesterase superfamily member 2, Acyl-coenzyme A thioesterase 13, N-terminally processed, ACOT13, THEM2 |
Target/Specificity | Human ACOT13 / THEM2 |
Reconstitution & Storage | Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C. |
Precautions | ACOT13 / THEM2 Antibody (aa100-150) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ACOT13 (HGNC:20999) |
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Synonyms | THEM2 |
Function | Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:16934754, PubMed:19170545). Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates (By similarity) (PubMed:16934754, PubMed:19170545). Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro) (By similarity) (PubMed:16934754, PubMed:19170545). May play a role in controlling adaptive thermogenesis (By similarity). |
Cellular Location | Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9CQR4}. Mitochondrion {ECO:0000250|UniProtKB:Q9CQR4}. Nucleus {ECO:0000250|UniProtKB:Q9CQR4} Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:Q9CQR4} Note=During interphase, found both in the nucleus and in the cytoplasm At mitosis, localizes to the spindle. Colocalizes with tubulin {ECO:0000250|UniProtKB:Q9CQR4} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis (By similarity).
References
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Yu W.-Q.,et al.Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).
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