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> home > Products > Primary Antibodies > Signal Transduction > ALOX5 / 5-Lipoxygenase Antibody (aa246-295)
ALOX5 / 5-Lipoxygenase Antibody (aa246-295)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P09917 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 78kDa |
| Dilution | ELISA (1:10000), IHC-P (5 µg/ml), WB (1:500-1:1000) |
| Gene ID | 240 |
|---|---|
| Other Names | Arachidonate 5-lipoxygenase, 5-LO, 5-lipoxygenase, 1.13.11.34, ALOX5, LOG5 |
| Target/Specificity | Arachidonate 5 Lipoxygenase (Ab-271) Antibody detects endogenous levels of total Arachidonate 5 Lipoxygenase protein. |
| Reconstitution & Storage | Store at -20°C for up to one year. |
| Precautions | ALOX5 / 5-Lipoxygenase Antibody (aa246-295) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ALOX5 (HGNC:435) |
|---|---|
| Synonyms | LOG5 |
| Function | Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)- eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation (PubMed:8631361, PubMed:21233389, PubMed:22516296, PubMed:24282679, PubMed:19022417, PubMed:23246375, PubMed:8615788, PubMed:24893149, PubMed:31664810). Also catalyzes the oxygenation of arachidonate into 8- hydroperoxyicosatetraenoate (8-HPETE) and 12- hydroperoxyicosatetraenoate (12-HPETE) (PubMed:23246375). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene (PubMed:31664810). Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses (PubMed:21206090, PubMed:31664810, PubMed:8615788, PubMed:17114001, PubMed:32404334). Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma) (By similarity). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers (PubMed:31664810). In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes (By similarity). Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40 (PubMed:21200133). May also play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK (By similarity). Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells (By similarity). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P48999, ECO:0000269|PubMed:18978352}. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Cytoplasm, perinuclear region. Cytoplasm, cytosol. Nucleus envelope. Nucleus intermembrane space. Note=Shuttles between cytoplasm and nucleus (PubMed:19233132). Found exclusively in the nucleus, when phosphorylated on Ser-272 (PubMed:18978352). Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association (PubMed:19233132, PubMed:3118366, PubMed:8245774, PubMed:16275640). |
| Volume | 50 µl |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
References
Dixon R.A.F.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:416-420(1988).
Matsumoto T.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:26-30(1988).
Matsumoto T.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:3406-3406(1988).
Matsumoto T.,et al.Adv. Prostaglandin Thromboxane Leukotriene Res. 19:466-469(1989).
Boudreau L.H.,et al.FASEB J. 25:1097-1105(2011).
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