ARFIP1 Antibody (aa271-320)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
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Primary Accession | P53367 |
Other Accession | 27236 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 41738 Da |
Dilution | ELISA (1:40000), IF (1:100 - 1:500), IHC-P (20 µg/ml), WB (1:500 - 1:1000), |
Gene ID | 27236 |
---|---|
Other Names | ARFIP1, Arfaptin-1, Arfaptin 1, HSU52521 |
Target/Specificity | ARFIP1 Antibody detects endogenous levels of total ARFIP1 protein. |
Reconstitution & Storage | PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year. |
Precautions | ARFIP1 Antibody (aa271-320) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ARFIP1 {ECO:0000303|PubMed:22981988, ECO:0000312|HGNC:HGNC:21496} |
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Function | Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network (PubMed:22981988). Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold (PubMed:9038142, PubMed:22981988). Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs (PubMed:22981988). In turn, ARFs induce fission (PubMed:22981988). Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells (PubMed:22981988). |
Cellular Location | Golgi apparatus. Golgi apparatus, trans-Golgi network membrane |
Tissue Location | Ubiquitously expressed (PubMed:9038142). Higher levels in liver, pancreas, placenta, skeletal muscle and heart (PubMed:9038142). |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Putative target protein of ADP-ribosylation factor.
References
Kanoh H.,et al.J. Biol. Chem. 272:5421-5429(1997).
Premont R.T.,et al.Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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