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CAPN6 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - CAPN6 Antibody AM8453b
    Western blot analysis of lysate from human placenta tissue lysate, using CAPN6 Antibody(Cat. #AM8453b). AM8453b was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20μg.
    detail
  • FC - CAPN6 Antibody AM8453b
    Flow cytometric analysis of A549 cells using CAPN6 Antibody(green, Cat#) compared to an isotype control of mouse IgG1(blue). was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9Y6Q1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone/Animal Names 1395CT446.22.5
Calculated MW 74576 Da
Additional Information
Gene ID 827
Other Names Calpain-6, Calpain-like protease X-linked, Calpamodulin, CalpM, CAPN6, CALPM, CANPX
Target/Specificity This CAPN6 antibody is generated from a mouse immunized with a recombinant protein of human CAPN6.
Dilution WB~~1:1000
FC~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAPN6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CAPN6
Synonyms CALPM, CANPX
Function Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues (By similarity).
Cellular Location Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, spindle. Note=During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle
Tissue Location Expressed only in placenta.
Research Areas
Citations (0)
citation

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Background

Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues (By similarity).

References

Dear T.N.,et al.Genomics 45:175-184(1997).
Crotty P.L.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
Belsito A.,et al.Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
Ross M.T.,et al.Nature 434:325-337(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AM8453b
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