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SLC27A5 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - SLC27A5 Antibody AO2264a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - SLC27A5 Antibody AO2264a
    Figure 1: Western blot analysis using SLC27A5 mAb against human SLC27A5 recombinant protein. (Expected MW is 32.9 kDa)
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  • WB - SLC27A5 Antibody AO2264a
    Figure 2: Western blot analysis using SLC27A5 mAb against HEK293 (1) and SLC27A5 (AA: 508-570)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - SLC27A5 Antibody AO2264a
    Figure 3: Western blot analysis using SLC27A5 mouse mAb against 3T3L1 (1), HepG2 (2), U937 (3), Raji (4), COS7 (5), NIH3T3 (6), and PC-3 (7) cell lysate.
    detail
  • IHC - SLC27A5 Antibody AO2264a
    Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using SLC27A5 mouse mAb with DAB staining.
    detail
  • IHC - SLC27A5 Antibody AO2264a
    Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SLC27A5 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q9Y2P5
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 9C4D1
Isotype IgG1
Calculated MW 75.4kDa
Description The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog.
Immunogen Purified recombinant fragment of human SLC27A5 (AA: 508-570 ) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 10998
Other Names Bile acyl-CoA synthetase, BACS, 6.2.1.7, Bile acid-CoA ligase, BA-CoA ligase, BAL, Cholate--CoA ligase, Fatty acid transport protein 5, FATP-5, Fatty-acid-coenzyme A ligase, very long-chain 3, Solute carrier family 27 member 5, Very long-chain acyl-CoA synthetase homolog 2, VLCS-H2, VLCSH2, Very long-chain acyl-CoA synthetase-related protein, VLACS-related, VLACSR, SLC27A5, ACSB, ACSVL6, FACVL3, FATP5
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC27A5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC27A5
Synonyms ACSB, ACSVL6, FACVL3, FATP5
Function May mediate the import of long-chain fatty acids (LCFA) by facilitating their transport across cell membranes (PubMed:20448275, PubMed:20530735). Also catalyzes the ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates (PubMed:10479480). Mainly functions as a bile acyl-CoA synthetase catalyzing the activation of bile acids via ATP-dependent formation of bile acid CoA thioesters which is necessary for their subsequent conjugation with glycine or taurine (PubMed:10749848, PubMed:11980911). Both primary bile acids (cholic acid and chenodeoxycholic acid) and secondary bile acids (deoxycholic acid and lithocholic acid) are the principal substrates (PubMed:10749848, PubMed:11980911). In vitro, activates 3-alpha,7-alpha,12-alpha- trihydroxy-5-beta-cholestanate ((25R)-3alpha,7alpha,12alpha-trihydroxy- 5beta-cholestan-26-oate or THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol (PubMed:11980911). Plays an important role in hepatic fatty acid uptake and bile acid reconjugation and recycling but not in de novo synthesis of bile acids (By similarity).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome {ECO:0000250|UniProtKB:Q9ES38}. Cell membrane {ECO:0000250|UniProtKB:Q4LDG0}; Multi-pass membrane protein
Tissue Location Predominantly expressed in liver.
Research Areas
Citations (0)
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References

1.Horm Metab Res. 2010 Nov;42(12):854-9. 2.Mol Nutr Food Res. 2007 Feb;51(2):185-91.

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$ 385.00
Cat# AO2264a
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