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BCL2L2

Purified Mouse Monoclonal Antibody

     
  • E - BCL2L2 AO2602a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - BCL2L2 AO2602a
    Figure 2:Western blot analysis using BCL2L2 mAb against human BCL2L2 (AA: 6-118) recombinant protein. (Expected MW is 38.2 kDa)
    detail
  • WB - BCL2L2 AO2602a
    Figure 3:Western blot analysis using BCL2L2 mAb against HEK293 (1) and BCL2L2 (AA: 6-118)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FCM - BCL2L2 AO2602a
    Figure 4:Flow cytometric analysis of Hela cells using BCL2L2 mouse mAb (green) and negative control (red).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession Q92843
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4G12D11
Isotype Mouse IgG2a
Calculated MW 20.7kDa
Immunogen Purified recombinant fragment of human BCL2L2 (AA: 6-118) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 599
Other Names BCLW; BCL-W; PPP1R51; BCL2-L-2
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
FCM~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCL2L2 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL2L2
Synonyms BCLW, KIAA0271
Function Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
Cellular Location Mitochondrion membrane; Peripheral membrane protein. Note=Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane
Tissue Location Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines
Research Areas
Citations (0)
citation

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References

1.J Mol Biol. 2014 Jun 12;426(12):2346-62.2.Zhonghua Zhong Liu Za Zhi. 2012 Mar;34(3):182-6.

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$ 385.00
Cat# AO2602a
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