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CD114

Purified Mouse Monoclonal Antibody

     
  • E - CD114 AO2717a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - CD114 AO2717a
    Figure 2:Western blot analysis using CD114 mAb against human CD114 (AA: extra 25-187) recombinant protein. (Expected MW is 46.5 kDa)
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  • WB - CD114 AO2717a
    Figure 3:Western blot analysis using CD114 mAb against HEK293 (1) and CD114 (AA: extra 25-187)-hIgGFc transfected HEK293 (2) cell lysate.
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  • FCM - CD114 AO2717a
    Figure 4:Flow cytometric analysis of K562 cells using CD114 mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession Q99062
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3G10G2
Isotype Mouse IgG1
Calculated MW 92.2kDa
Immunogen Purified recombinant fragment of human CD114 (AA: extra 25-187) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1441
Other Names CSF3R; SCN7; GCSFR
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
FCM~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD114 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CSF3R
Synonyms GCSFR
Function Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
Cellular Location [Isoform 2]: Secreted.
Tissue Location One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta
Research Areas
Citations (0)
citation

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References

1.Clin Cancer Res. 2016 Feb 1;22(3):757-64.2.World J Gastroenterol. 2014 Jan 28;20(4):1074-8.

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$ 385.00
Cat# AO2717a
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