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PPP1R3A Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPP1R3A Antibody (Center) AP10676C
    PPP1R3A Antibody (Center) (Cat. #AP10676c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PPP1R3A antibody detected the PPP1R3A protein (arrow).
    detail
  • FC - PPP1R3A Antibody (Center) AP10676C
    PPP1R3A Antibody (Center) (Cat. #AP10676c) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q16821
Other Accession Q00756, NP_002702.2
Reactivity Human
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 125767 Da
Antigen Region 754-782 aa
Additional Information
Gene ID 5506
Other Names Protein phosphatase 1 regulatory subunit 3A, Protein phosphatase 1 glycogen-associated regulatory subunit, Protein phosphatase type-1 glycogen targeting subunit, RG1, PPP1R3A, PP1G
Target/Specificity This PPP1R3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-782 amino acids from the Central region of human PPP1R3A.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPP1R3A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP1R3A
Synonyms PP1G
Function Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Plays an important role in glycogen synthesis but is not essential for insulin activation of glycogen synthase (By similarity).
Cellular Location Membrane; Single-pass membrane protein
Tissue Location Skeletal muscle and heart.
Research Areas
Citations (0)
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Background

The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase. [provided by RefSeq].

References

Yoshida, T., et al. Int. J. Mol. Med. 24(2):233-246(2009)
Yoshida, T., et al. Genomics 93(3):221-226(2009)
Gieger, C., et al. PLoS Genet. 4 (11), E1000282 (2008) :
Savage, D.B., et al. PLoS Med. 5 (1), E27 (2008) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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$ 182.50
$ 70.00
Cat# AP10676C
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