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CALCA Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CALCA Antibody (Center) AP11119c
    Anti-CALCA Antibody (Center) at 1:2000 dilution + TT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IHC-P - CALCA Antibody (Center) AP11119c
    CALCA Antibody (Center) (Cat. #AP11119c)immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CALCA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P06881
Other Accession NP_001029125.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 13899 Da
Antigen Region 56-83 aa
Additional Information
Gene ID 796
Other Names Calcitonin gene-related peptide 1, Alpha-type CGRP, Calcitonin gene-related peptide I, CGRP-I, CALCA, CALC1
Target/Specificity This CALCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the Central region of human CALCA.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCALCA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CALCA
Synonyms CALC1
Function CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.
Cellular Location Secreted.
Research Areas
Citations (0)
citation

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Background

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.

References

Giroux, S., et al. Bone 47(5):975-981(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Lee, J.Y., et al. Korean J Lab Med 30(4):406-413(2010)
Giovanella, L., et al. Clin. Chem. Lab. Med. 48(8):1163-1165(2010)
Hatzistilianou, M. ScientificWorldJournal 10, 1941-1946 (2010) :

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$ 182.50
$ 70.00
Cat# AP11119c
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