Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
ATG12 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IF, E |
|---|---|
| Primary Accession | O94817 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 15113 Da |
| Antigen Region | 8-38 aa |
| Gene ID | 9140 |
|---|---|
| Other Names | Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like, ATG12, APG12, APG12L |
| Target/Specificity | This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-38 amino acids from the N-terminal region of human ATG12. |
| Dilution | IF~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ATG12 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATG12 (HGNC:588) |
|---|---|
| Synonyms | APG12, APG12L |
| Function | Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. |
| Cellular Location | Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=TECPR1 recruits the ATG12- ATG5 conjugate to the autolysosomal membrane |
| Tissue Location | Ubiquitous.. |
Provided below are standard protocols that you may find useful for product applications.
Background
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.
References
Tanida, I., et al., Biochem. Biophys. Res. Commun. 296(5):1164-1170 (2002).
Tanida, I., et al., J. Biol. Chem. 277(16):13739-13744 (2002).
Mizushima, N., et al., J. Biol. Chem. 273(51):33889-33892 (1998).
Ueno, K., et al., Hum. Genet. 102(1):63-68 (1998).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
