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CPB1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - CPB1 Antibody (N-term) AP12023a
    Confocal immunofluorescent analysis of CPB1 Antibody (N-term)(Cat#AP12023a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - CPB1 Antibody (N-term) AP12023a
    CPB1 Antibody (N-term) (Cat. #AP12023a) western blot analysis in K562,Hela cell line lysates (35ug/lane).This demonstrates the CPB1 antibody detected the CPB1 protein (arrow).
    detail
  • WB - CPB1 Antibody (N-term) AP12023a
    Anti-CPB1 Antibody (N-term) at 1:1000 dilution + human pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CPB1 Antibody (N-term) AP12023a
    CPB1 Antibody (N-term) (Cat. #AP12023a)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPB1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - CPB1 Antibody (N-term) AP12023a
    CPB1 Antibody (N-term) (Cat. #AP12023a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P15086
Other Accession NP_001862.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 47368 Da
Antigen Region 9-37 aa
Additional Information
Gene ID 1360
Other Names Carboxypeptidase B, Pancreas-specific protein, PASP, CPB1, CPB, PCPB
Target/Specificity This CPB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human CPB1.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCPB1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CPB1
Synonyms CPB, PCPB
Cellular Location Secreted {ECO:0000250|UniProtKB:P00732}. Zymogen granule lumen {ECO:0000250|UniProtKB:P55261}
Tissue Location Pancreas..
Research Areas
Citations (0)
citation

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Background

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.

References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Pantoja-Uceda, D., et al. Biochemistry 47(27):7066-7078(2008)
Guimaraes, A.H., et al. Arterioscler. Thromb. Vasc. Biol. 27(10):2157-2162(2007)
Barbosa Pereira, P.J., et al. J. Mol. Biol. 321(3):537-547(2002)
Aloy, P., et al. Biol. Chem. 379(2):149-155(1998)

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$ 365.00
$ 140.00
Cat# AP12023a
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