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JHDM2b Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - JHDM2b Antibody (Center) AP12070c
    JHDM2b Antibody (Center) (Cat. #AP12070c) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the JHDM2b antibody detected the JHDM2b protein (arrow).
    detail
  • FC - JHDM2b Antibody (Center) AP12070c
    JHDM2b Antibody (Center) (Cat. #AP12070c) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q7LBC6
Other Accession Q6ZPY7, NP_057688.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 191581 Da
Antigen Region 869-897 aa
Additional Information
Gene ID 51780
Other Names Lysine-specific demethylase 3B, 11411-, JmjC domain-containing histone demethylation protein 2B, Jumonji domain-containing protein 1B, Nuclear protein 5qNCA, KDM3B, C5orf7, JHDM2B, JMJD1B, KIAA1082
Target/Specificity This JHDM2b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 869-897 amino acids from the Central region of human JHDM2b.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsJHDM2b Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM3B
Synonyms C5orf7, JHDM2B, JMJD1B, KIAA1082
Function Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
Cellular Location Nucleus.
Tissue Location Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.
Research Areas
Citations (0)
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Background

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Yamane, K., et al. Cell 125(3):483-495(2006)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)

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$ 365.00
$ 140.00
Cat# AP12070c
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