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NEU2 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| IHC-P-Leica, FC, IF, WB, E |
|---|---|
| Primary Accession | Q9Y3R4 |
| Other Accession | NP_005374.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 42254 Da |
| Antigen Region | 23-50 aa |
| Gene ID | 4759 |
|---|---|
| Other Names | Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2, NEU2 |
| Target/Specificity | This NEU2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-50 amino acids from the N-terminal region of human NEU2. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P-Leica~~1:250 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NEU2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NEU2 |
|---|---|
| Function | Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides (PubMed:14613940, PubMed:22228546). Recognizes sialyl linkage positions of the glycan moiety as well as the supramolecular organization of the sialoglycoconjugate. Displays preference for alpha- (2->3)-sialylated GD1a and GT1B gangliosides over alpha-(2->8)- sialylated GD1b, in both monomeric forms and micelles. Hydrolyzes monomeric GM1 ganglioside, but has no activity toward the miscellar form (PubMed:14613940). Has lower sialidase activity for glycoproteins such as fetuin and TF/transferrin that carry a mixture of alpha-(2->3) and alpha-(2->6)-sialyl linkages. Cleaves milk oligosaccharide alpha- (2->3)-sialyllactose, but is inactive toward alpha-(2->6)-sialyllactose isomer. Has no activity toward colominic acid, a homomer of alpha- (2->8)-linked Neu5Ac residues (PubMed:14613940). |
| Cellular Location | Cytoplasm, cytosol. |
| Tissue Location | Expressed in skeletal muscle, fetal liver and embryonic carcinoma cell line NT2-D1. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq].
References
Stoppani, E., et al. Cell Biol. Int. 33(9):1020-1025(2009)
Li, C.Y., et al. Cell Res. 17(4):357-362(2007)
Chavas, L.M., et al. J. Biol. Chem. 280(1):469-475(2005)
Seyrantepe, V., et al. J. Biol. Chem. 279(35):37021-37029(2004)
Tringali, C., et al. J. Biol. Chem. 279(5):3169-3179(2004)
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