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ARHB Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P62745 |
| Other Accession | P62747, P62746, Q3ZBW5, NP_004031.1 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 22123 Da |
| Antigen Region | 104-137 aa |
| Gene ID | 388 |
|---|---|
| Other Names | Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6, RHOB, ARH6, ARHB |
| Target/Specificity | This ARHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-137 amino acids from the Central region of human ARHB. |
| Dilution | WB~~1:4000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ARHB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RHOB |
|---|---|
| Synonyms | ARH6, ARHB |
| Function | Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells. |
| Cellular Location | Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Cleavage furrow. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus Translocates to the equatorial region before furrow formation in a ECT2-dependent manner |
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Provided below are standard protocols that you may find useful for product applications.
Background
ARHB mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
References
Adly, M.A., et al. J. Cutan. Pathol. 37(7):751-757(2010)
Connolly, E.C., et al. Mol. Cancer Res. 8(5):691-700(2010)
Zintzaras, E., et al. Am. J. Epidemiol. 171(8):851-858(2010)
Kim, C.H., et al. Biochem. Biophys. Res. Commun. 391(2):1182-1186(2010)
Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010)
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