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SENP3 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9H4L4 |
| Other Accession | Q9EP97 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 65010 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 26168 |
|---|---|
| Other Names | Sentrin-specific protease 3, SUMO-1-specific protease 3, Sentrin/SUMO-specific protease SENP3, SENP3, SSP3, SUSP3 |
| Target/Specificity | This SENP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SENP3. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SENP3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SENP3 |
|---|---|
| Function | Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates (PubMed:16608850, PubMed:32832608). Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability (PubMed:15743823). Deconjugates SUMO2 and SUMO3 from CDCA8 (PubMed:18946085). Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300 (PubMed:19680224). Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1 (PubMed:19015314). Plays a role in the regulation of sumoylation status of ZNF148 (PubMed:18259216). Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed:22872859). Deconjugates SUMO2 from KAT5 (PubMed:32832608). |
| Cellular Location | Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm {ECO:0000250|UniProtKB:Q9EP97} Note=Redistributes between the nucleolus and the nucleoplasm in response to mild oxidative stress (PubMed:19680224). Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). {ECO:0000250|UniProtKB:Q9EP97, ECO:0000269|PubMed:19680224} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
SENP3 releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. This protein deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability.
References
Muller S, et al., Nat Rev Mol Cell Biol. 2001 2(3):202-10 Review.
Hochstrasser M. Cell. 2001 107(1):5-8. Review.
Kahyo T, et al., Mol Cell. 2001 Sep;8(3):713-8.
Yeh ET, et al., Gene. 2000 May 2;248(1-2):1-14. Review.
Keane,M.M., et al., Oncogene 18 (22), 3365-3375 (1999)
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