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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   UGT2B15 Antibody (Center)   

UGT2B15 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - UGT2B15 Antibody (Center) AP12375C
    Confocal immunofluorescent analysis of UGT2B15 Antibody (Center) (Cat#AP12375c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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  • WB - UGT2B15 Antibody (Center) AP12375C
    Anti-UGT2B15 Antibody (Center) at 1:500 dilution + Human liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - UGT2B15 Antibody (Center) AP12375C
    Immunohistochemical analysis of AP12375C on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - UGT2B15 Antibody (Center) AP12375C
    UGT2B15 Antibody (Center) (Cat. #AP12375c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, FC, IF, E
Primary Accession P54855
Other Accession NP_001067.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 61036 Da
Antigen Region 156-185 aa
Additional Information
Gene ID 7366
Other Names UDP-glucuronosyltransferase 2B15, UDPGT 2B15, HLUG4, UDP-glucuronosyltransferase 2B8, UDPGT 2B8, UDPGTh-3, UGT2B15, UGT2B8
Target/Specificity This UGT2B15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the Central region of human UGT2B15.
Dilution IF~~1:10~50
WB~~1:500
IHC-P~~1:100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUGT2B15 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UGT2B15 (HGNC:12546)
Function UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed:7835232, PubMed:9295060, PubMed:16595710, PubMed:18719240, PubMed:23288867). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed:7835232). Catalyzes the glucuronidation of endogenous steroid hormones such as androgens (testosterone, androsterone) and estrogens (estradiol, epiestradiol, estriol, catechol estrogens) (PubMed:7835232, PubMed:9295060, PubMed:16595710, PubMed:18719240, PubMed:23288867). Displays glucuronidation activity toward several classes of xenobiotic substrates, including phenolic compounds (eugenol, 4-nitrophenol, 4- hydroxybiphenyl) and phenylpropanoids (naringenin, coumarins) (PubMed:7835232). Catalyzes the glucuronidation of monoterpenoid alcohols such as borneol, menthol and isomenthol, a class of natural compounds used in essential oils (By similarity).
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location Expressed in many tissues. Present in liver, prostate and testis.
Research Areas
Citations ( 0 )

Background

The UGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. UGT2B8 demonstrates reactivity with estriol. See UGT2B4 (MIM 600067).

References

Yong, M., et al. Cancer Epidemiol. Biomarkers Prev. 19(2):537-546(2010)
Sun, C., et al. Hum. Mutat. 31(1):99-107(2010)
Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
He, X., et al. Br J Clin Pharmacol 68(5):721-730(2009)
Holmes, M.V., et al. PLoS ONE 4 (12), E7960 (2009) :

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$ 182.50
$ 70.00
Cat# AP12375C
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