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SENP5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SENP5 Antibody (C-term) AP1237a
    Western blot analysis of SENP5 polyclonal antibody (Cat. #AP1237a) in Saos-2 cell lysate. SENP5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
    detail
  • IHC-P - SENP5 Antibody (C-term) AP1237a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
  • IHC-P - SENP5 Antibody (C-term) AP1237a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SENP5 Antibody (C-term) (Cat.#AP1237a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q96HI0
Other Accession Q6NXL6, Q8WP32
Reactivity Human
Predicted Monkey, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 86693 Da
Antigen Region 524-555 aa
Additional Information
Gene ID 205564
Other Names Sentrin-specific protease 5, Sentrin/SUMO-specific protease SENP5, SENP5
Target/Specificity This SENP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 524-555 amino acids from the C-terminal region of human SENP5.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSENP5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SENP5
Function Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division.
Cellular Location Nucleus, nucleolus
Research Areas
Citations ( 0 )

Background

SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its conjugatable mature form and deconjugation of SUMO2 and SUMO3 from targeted substrates. This protein has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. SENP5 is required for cell division.

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).

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$ 182.50
$ 70.00
Cat# AP1237a
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