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GBP1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GBP1 Antibody (N-term) AP12492A
    GBP1 Antibody (N-term) (Cat. #AP12492a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GBP1 antibody detected the GBP1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P32455
Other Accession NP_002044.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 67931 Da
Antigen Region 141-170 aa
Additional Information
Gene ID 2633
Other Names Interferon-induced guanylate-binding protein 1, GTP-binding protein 1, GBP-1, HuGBP-1, Guanine nucleotide-binding protein 1, GBP1
Target/Specificity This GBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-170 amino acids from the N-terminal region of human GBP1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGBP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GBP1
Function Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens (PubMed:22106366, PubMed:29144452, PubMed:16511497, PubMed:31268602). Hydrolyzes GTP to GMP in two consecutive cleavage reactions: GTP is first hydrolyzed to GDP and then to GMP in a processive manner (PubMed:7512561, PubMed:16511497). Following infection, recruited to the pathogen-containing vacuoles or vacuole- escaped bacteria and promotes both autophagy and inflammasome assembly (PubMed:29144452, PubMed:31268602). Promotes host defense against bacterial infections by regulating bacteriolytic peptide generation via its interaction with ubiquitin-binding protein SQSTM1, which delivers monoubiquitinated proteins to autolysosomes for the generation of bacteriolytic peptides (By similarity). Also acts as a positive regulator of inflammasome assembly by promoting the release of inflammasome ligands from bacteria (PubMed:31268602). Acts by promoting lysis of pathogen-containing vacuoles, releasing pathogens into the cytosol (By similarity). Following pathogen release in the cytosol, promotes recruitment of proteins that mediate bacterial cytolysis: this liberates ligands that are detected by inflammasomes, such as lipopolysaccharide (LPS) that activates the non-canonical CASP4/CASP11 inflammasome or double-stranded DNA (dsDNA) that activates the AIM2 inflammasome (PubMed:31268602). Confers protection to several pathogens, including the bacterial pathogens L.monocytogenes and M.bovis BCG as well as the protozoan pathogen T.gondii (PubMed:31268602). Exhibits antiviral activity against influenza virus (PubMed:22106366).
Cellular Location Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm Secreted. Note=Localizes to pathogen- containing vacuoles or to the cell surface of bacteria that escaped vacuoles (PubMed:29144452, PubMed:31268602). Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of IFNG induction (PubMed:16936281). Golgi membrane localization requires isoprenylation and the presence of another IFNG- induced factor (PubMed:15937107).
Research Areas
Citations (0)
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Background

Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3.

References

Vopel, T., et al. J. Mol. Biol. 400(1):63-70(2010)
Mirpuri, J., et al. J. Immunol. 184(12):7186-7195(2010)
Lipnik, K., et al. Mol. Med. 16 (5-6), 177-187 (2010) :
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
O'Doherty, C., et al. Pharmacogenomics 10(7):1177-1186(2009)

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$ 182.50
$ 70.00
Cat# AP12492A
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