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WDR3 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - WDR3 Antibody (N-term) AP12525a
    WDR3 Antibody (N-term) (Cat. #AP12525a) western blot analysis in Hela,K562 cell line lysates (35ug/lane).This demonstrates the WDR3 antibody detected the WDR3 protein (arrow).
    detail
  • FC - WDR3 Antibody (N-term) AP12525a
    WDR3 Antibody (N-term) (Cat. #AP12525a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q9UNX4
Other Accession NP_006775.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 106099 Da
Antigen Region 208-236 aa
Additional Information
Gene ID 10885
Other Names WD repeat-containing protein 3, WDR3
Target/Specificity This WDR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-236 amino acids from the N-terminal region of human WDR3.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWDR3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR3 (HGNC:12755)
Function Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre- rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre- ribosomal RNA by the RNA exosome.
Cellular Location Nucleus, nucleolus
Tissue Location Ubiquitous.
Research Areas
Citations (0)
citation

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Background

This gene encodes a nuclear protein containing 10 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, which usually include a trp-asp at the C-terminal end. Proteins belonging to the WD repeat family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation.

References

Akdi, A., et al. Thyroid 20(7):803-809(2010)
McMahon, M., et al. J. Biol. Chem. 285(24):18309-18318(2010)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)

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$ 365.00
$ 140.00
Cat# AP12525a
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