SLC8A3 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | P57103 |
Other Accession | NP_892114.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 103010 Da |
Antigen Region | 585-614 aa |
Gene ID | 6547 |
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Other Names | Sodium/calcium exchanger 3, Na(+)/Ca(2+)-exchange protein 3, Solute carrier family 8 member 3, SLC8A3, NCX3 |
Target/Specificity | This SLC8A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 585-614 amino acids of human SLC8A3. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SLC8A3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SLC8A3 |
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Synonyms | NCX3 |
Function | Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells, both in muscle and in brain. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A3 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline. Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In neurons, contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory (By similarity). Required for normal oligodendrocyte differentiation and for normal myelination (PubMed:21959935). Mediates Ca(2+) efflux from mitochondria and contributes to mitochondrial Ca(2+) ion homeostasis (By similarity). |
Cellular Location | Cell membrane; Multi-pass membrane protein. Perikaryon {ECO:0000250|UniProtKB:P70549}. Cell projection, dendrite {ECO:0000250|UniProtKB:P70549}. Cell projection, dendritic spine {ECO:0000250|UniProtKB:P70549}. Cell membrane, sarcolemma {ECO:0000250|UniProtKB:S4R2P9}. Cytoplasm, sarcoplasm {ECO:0000250|UniProtKB:S4R2P9}. Cell junction {ECO:0000250|UniProtKB:S4R2P9}. Mitochondrion outer membrane {ECO:0000250|UniProtKB:S4R2P9}; Multi-pass membrane protein {ECO:0000250|UniProtKB:S4R2P9}. Cytoplasm, perinuclear region. Endoplasmic reticulum membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:S4R2P9}. Note=Detected at neuromuscular junctions. {ECO:0000250|UniProtKB:S4R2P9} |
Tissue Location | Isoform 2 is expressed in brain and skeletal muscle. Isoform 3 is expressed in excitable cells of brain, retina and skeletal muscle. Isoform 4 is expressed in skeletal muscle |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the sodium/calcium exchanger integral membrane protein family. Three mammalian isoforms in family 8 have been identified. Na+/Ca2+ exchange proteins are involved in maintaining Ca2+ homeostasis in a wide variety of cell types. The protein is regulated by intracellular calcium ions and is found in both the plasma membrane and intracellular organellar membranes, where exchange of Na+ for Ca2+ occurs in an electrogenic manner. Alternative splicing has been observed for this gene and multiple variants have been described.
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Pulina, M.V., et al. J. Biol. Chem. 281(28):19645-19654(2006)
Gomez-Villafuertes, R., et al. J. Neurosci. 25(47):10822-10830(2005)
Lindgren, R.M., et al. Gene 348, 143-155 (2005) :
Gabellini, N. Mol. Neurobiol. 30(1):91-116(2004)
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