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DCTN2 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q13561 |
| Other Accession | Q6AYH5, Q99KJ8, Q3ZCF0, NP_006391.1, A0A5G2QD80 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 44231 Da |
| Antigen Region | 192-219 aa |
| Gene ID | 10540 |
|---|---|
| Other Names | Dynactin subunit 2, 50 kDa dynein-associated polypeptide, Dynactin complex 50 kDa subunit, DCTN-50, p50 dynamitin, DCTN2, DCTN50 |
| Target/Specificity | This DCTN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-219 amino acids from the Central region of human DCTN2. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DCTN2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DCTN2 (HGNC:2712) |
|---|---|
| Synonyms | DCTN50 |
| Function | Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In the dynactin soulder domain, binds the ACTR1A filament and acts as a molecular ruler to determine the length (By similarity). Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development (By similarity). |
| Cellular Location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:A0A5G2QD80} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.
References
Jacquot, G., et al. J. Biol. Chem. 285(30):23019-23031(2010)
Inoue, M., et al. Genes Cells 13(8):905-914(2008)
Maier, K.C., et al. Traffic 9(4):481-491(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Camargo, L.M., et al. Mol. Psychiatry 12(1):74-86(2007)
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