PKP2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | Q99959 |
Other Accession | NP_004563.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 97415 Da |
Antigen Region | 838-866 aa |
Gene ID | 5318 |
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Other Names | Plakophilin-2, PKP2 |
Target/Specificity | This PKP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 838-866 amino acids from the C-terminal region of human PKP2. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PKP2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PKP2 (HGNC:9024) |
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Function | Regulates focal adhesion turnover resulting in changes in focal adhesion size, cell adhesion and cell spreading, potentially via transcriptional modulation of beta-integrins (PubMed:23884246). Required to maintain gingival epithelial barrier function (PubMed:34368962). Required for cardiac sodium current propagation and electrical synchrony in cardiac myocytes (By similarity). Required for the formation of desmosome cell junctions in cardiomyocytes, thereby required for the correct formation of the heart, specifically trabeculation and formation of the atria walls (By similarity). Loss of desmosome cell junctions leads to mis-localization of DSP and DSG2 resulting in disruption of cell-cell adhesion and disordered intermediate filaments (By similarity). Modulates profibrotic gene expression in cardiomyocytes via regulation of DSP expression and subsequent activation of downstream TGFB1 and MAPK14/p38 MAPK signaling (By similarity). May play a role in junctional plaques (PubMed:22781308). Involved in the inhibition of viral infection by influenza A viruses (IAV) (PubMed:28169297). Acts as a host restriction factor for IAV viral propagation, potentially via disrupting the interaction of IAV polymerase complex proteins (PubMed:28169297). |
Cellular Location | Nucleus. Cell junction, desmosome. Cell junction. Cytoplasm Note=Colocalizes with CTNNA3 and SCN5A/Nav1.5 at intercalated disks in the heart. {ECO:0000250|UniProtKB:Q9CQ73} |
Tissue Location | Detected in heart right ventricle (at protein level). Expressed in gingival epithelial, endothelial and fibroblast cells (at protein level) (PubMed:34368962). Faintly expressed in tracheal epithelial cells (at protein level) (PubMed:28169297). Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata |
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Background
This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13.
References
Fressart, V., et al. Europace 12(6):861-868(2010)
Cox, M.G., et al. Circ Arrhythm Electrophysiol 3(2):126-133(2010)
Christensen, A.H., et al. Cardiology 115(2):148-154(2010)
den Haan, A.D., et al. Circ Cardiovasc Genet 2(5):428-435(2009)
Bhuiyan, Z.A., et al. Circ Cardiovasc Genet 2(5):418-427(2009)
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