ARFIP1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | P53367 |
Other Accession | Q9JHU5, NP_055262.1, NP_001020766.1, G5E8V9 |
Reactivity | Human |
Predicted | Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 41738 Da |
Antigen Region | 147-176 aa |
Gene ID | 27236 |
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Other Names | Arfaptin-1, ADP-ribosylation factor-interacting protein 1, ARFIP1 |
Target/Specificity | This ARFIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-176 amino acids from the Central region of human ARFIP1. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ARFIP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ARFIP1 {ECO:0000303|PubMed:22981988, ECO:0000312|HGNC:HGNC:21496} |
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Function | Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network (PubMed:22981988). Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold (PubMed:9038142, PubMed:22981988). Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs (PubMed:22981988). In turn, ARFs induce fission (PubMed:22981988). Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells (PubMed:22981988). |
Cellular Location | Golgi apparatus. Golgi apparatus, trans-Golgi network membrane |
Tissue Location | Ubiquitously expressed (PubMed:9038142). Higher levels in liver, pancreas, placenta, skeletal muscle and heart (PubMed:9038142). |
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Provided below are standard protocols that you may find useful for product applications.
Background
ARFIP1 is a putative target protein of ADP-ribosylation factor.
References
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Foster, L.J., et al. J. Proteome Res. 5(1):64-75(2006)
Ho, W.T., et al. FEBS Lett. 537 (1-3), 91-95 (2003) :
Suzuki, Y., et al. Genome Res. 11(5):677-684(2001)
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