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SEMA4C Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SEMA4C Antibody (C-term) AP13003b
    All lanes : Anti-SEMA4C Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: U-87MG whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - SEMA4C Antibody (C-term) AP13003b
    Anti-SEMA4C Antibody (C-term) at 1:4000 dilution + Neuro-2a whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - SEMA4C Antibody (C-term) AP13003b
    SEMA4C Antibody (C-term) (Cat. #AP13003b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q9C0C4
Other Accession Q64151, NP_060259.4
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 92623 Da
Antigen Region 792-821 aa
Additional Information
Gene ID 54910
Other Names Semaphorin-4C, SEMA4C, KIAA1739, SEMAI
Target/Specificity This SEMA4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 792-821 amino acids of human SEMA4C.
Dilution WB~~1:4000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSEMA4C Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SEMA4C
Synonyms KIAA1739, SEMAI
Function Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration (By similarity). Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.
Cellular Location Postsynaptic density membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type I membrane protein
Research Areas
Citations (0)
citation

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Background

Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.

References

Wu, H., et al. Eur. J. Cell Biol. 86(6):331-344(2007)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Inagaki, S., et al. J. Biol. Chem. 276(12):9174-9181(2001)
Ohoka, Y., et al. Biochem. Biophys. Res. Commun. 280(1):237-243(2001)
Wang, L.H., et al. J. Biol. Chem. 274(20):14137-14146(1999)

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$ 365.00
$ 140.00
Cat# AP13003b
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