ZFYVE19 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | Q96K21 |
Other Accession | NP_001070736.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 51546 Da |
Antigen Region | 387-415 aa |
Gene ID | 84936 |
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Other Names | Abscission/NoCut checkpoint regulator, ANCHR, MLL partner containing FYVE domain, Zinc finger FYVE domain-containing protein 19, ZFYVE19, ANCHR, MPFYVE |
Target/Specificity | This ZFYVE19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of human ZFYVE19. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ZFYVE19 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ZFYVE19 |
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Synonyms | ANCHR, MPFYVE |
Function | Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission. |
Cellular Location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cleavage furrow. Midbody, Midbody ring. Note=Localizes mainly on centrosomes in interphase and early mitosis. Localizes at the cleavage furrow and midbody ring in late mitosis and cytokinesis |
Tissue Location | Detected in brain, heart, skeletal muscle and kidney (PubMed:12618766). Expressed in the liver (at protein level) (PubMed:12618766, PubMed:32737136). |
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Provided below are standard protocols that you may find useful for product applications.
References
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Chinwalla, V., et al. Oncogene 22(9):1400-1410(2003)
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