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CHST9 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CHST9 Antibody (N-term) AP13188a
    CHST9 Antibody (N-term) (Cat. #AP13188a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CHST9 antibody detected the CHST9 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7L1S5
Other Accession NP_113610.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52055 Da
Antigen Region 21-50 aa
Additional Information
Gene ID 83539
Other Names Carbohydrate sulfotransferase 9, 282-, GalNAc-4-O-sulfotransferase 2, GalNAc-4-ST2, GalNAc4ST-2, N-acetylgalactosamine-4-O-sulfotransferase 2, CHST9
Target/Specificity This CHST9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human CHST9.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHST9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHST9
Function Catalyzes the transfer of sulfate to position 4 of non- reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Participates in biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Has a higher activity toward carbonic anhydrase VI than toward lutropin. Only active against terminal GalNAcbeta1,GalNAcbeta. Isoform 2, but not isoform 1, is active toward chondroitin.
Cellular Location [Isoform 1]: Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Highly expressed in trachea. Also expressed in fetal lung, adult pancreas, testis and salivary gland. Expressed at low level in pituitary gland, apex of the heart, adult lung, prostate and mammary gland. Weakly or not expressed in heart, liver and spinal cord
Citations (0)
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Background

Sulfate groups in carbohydrates confer highly specific functions on glycoproteins, glycolipids, and proteoglycans and are critical for cell-cell interaction, signal transduction, and embryonic development. Sulfotransferases, such as CHST9, carry out sulfation of carbohydrates (Hiraoka et al., 2001 [PubMed 11445554]).

References

Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Hiraoka, N., et al. Glycobiology 11(6):495-504(2001)
Kang, H.G., et al. J. Biol. Chem. 276(14):10861-10869(2001)

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$ 182.50
$ 70.00
Cat# AP13188a
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