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RPL34 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - RPL34 Antibody (Center) AP13207C
    All lanes : Anti-RPL34 Antibody (Center) at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: U-251 MG whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: mouse brain lysate Lane 6: NIH3T3 whole cell lysate Lane 7: rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • WB - RPL34 Antibody (Center) AP13207C
    RPL34 Antibody (Center) (Cat. #AP13207c) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RPL34 antibody detected the RPL34 protein (arrow).
    detail
  • IHC-P - RPL34 Antibody (Center) AP13207C
    RPL34 Antibody (Center) (Cat. #AP13207c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL34 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P49207
Other Accession Q29223, Q9D1R9, NP_296374.1, NP_000986.2
Reactivity Human, Mouse, Rat
Predicted Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 13293 Da
Antigen Region 37-66 aa
Additional Information
Gene ID 6164
Other Names 60S ribosomal protein L34, RPL34
Target/Specificity This RPL34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-66 amino acids from the Central region of human RPL34.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL34 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL34
Function Component of the large ribosomal subunit (PubMed:12962325, PubMed:23636399, PubMed:25957688, PubMed:25901680, PubMed:32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:12962325, PubMed:23636399, PubMed:25957688, PubMed:25901680, PubMed:32669547).
Cellular Location Cytoplasm, cytosol. Cytoplasm Endoplasmic reticulum {ECO:0000250|UniProtKB:Q29223}. Note=Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity) {ECO:0000250|UniProtKB:Q29223, ECO:0000269|PubMed:25957688}
Research Areas
Citations ( 0 )

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Transcript variants derived from alternative splicing, alternative transcription initiation sites, and/or alternative polyadenylation exist; these variants encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

References

Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Mazumder, B., et al. Cell 115(2):187-198(2003)

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$ 365.00
$ 140.00
Cat# AP13207C
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