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RAB3D Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RAB3D Antibody (C-term) AP13216b
    RAB3D Antibody (C-term) (Cat. #AP13216b) western blot analysis in K562,HL-60,MDA-MB231,MDA-MB453,CEM cell line lysates (35ug/lane).This demonstrates the RAB3D antibody detected the RAB3D protein (arrow).
    detail
  • IHC-P - RAB3D Antibody (C-term) AP13216b
    RAB3D Antibody (C-term) (Cat. #AP13216b)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RAB3D Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - RAB3D Antibody (C-term) AP13216b
    RAB3D Antibody (C-term) (Cat. #AP13216b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O95716
Other Accession NP_004274.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 24267 Da
Antigen Region 185-212 aa
Additional Information
Gene ID 9545
Other Names Ras-related protein Rab-3D, RAB3D, GOV, RAB16
Target/Specificity This RAB3D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-212 amino acids from the C-terminal region of human RAB3D.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB3D Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB3D
Synonyms GOV, RAB16
Function Protein transport. Probably involved in regulated exocytosis (By similarity).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side
Tissue Location Highly expressed in granulocytes of peripheral blood. Constitutively expressed at low levels in all hematopoietic cell lines investigated
Research Areas
Citations (0)
citation

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Background

RAB3D is a protein transport. Probably involved in regulated exocytosis (By similarity).

References

Tian, X., et al. Mol. Cell. Biol. 30(5):1269-1284(2010)
Knop, M., et al. EMBO J. 23(15):2982-2992(2004)
Matsumoto, M., et al. Proc. Natl. Acad. Sci. U.S.A. 101(22):8313-8318(2004)
Nguyen, D., et al. J. Cell. Physiol. 197(3):400-408(2003)
Fukuda, M. J. Biol. Chem. 278(17):15373-15380(2003)

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$ 365.00
$ 140.00
Cat# AP13216b
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