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TMED9 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TMED9 Antibody (C-term) AP13250B
    TMED9 Antibody (C-term) (Cat. #AP13250b) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the TMED9 antibody detected the TMED9 protein (arrow).
    detail
  • IHC-P - TMED9 Antibody (C-term) AP13250B
    TMED9 Antibody (C-term) (Cat. #AP13250b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TMED9 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9BVK6
Other Accession Q5I0E7, Q99KF1, Q3T133, NP_059980.2
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 27277 Da
Antigen Region 169-198 aa
Additional Information
Gene ID 54732
Other Names Transmembrane emp24 domain-containing protein 9, GMP25, Glycoprotein 25L2, p24 family protein alpha-2, p24alpha2, p25, TMED9, GP25L2
Target/Specificity This TMED9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the C-terminal region of human TMED9.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTMED9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TMED9
Synonyms GP25L2
Function Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 isoform PTPB.
Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, cis-Golgi network membrane; Single-pass type I membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein Golgi apparatus, trans-Golgi network membrane; Single- pass type I membrane protein. Note=Cycles between compartments of the early secretatory pathway
Research Areas
Citations (0)
citation

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Background

The specific function of this protein remains unknown.

References

Breuza, L., et al. J. Biol. Chem. 279(45):47242-47253(2004)
Zhang, H., et al. Nat. Biotechnol. 21(6):660-666(2003)
Zhang, H., et al. Nat. Biotechnol. 21(6):660-666(2003)
Renz, M., et al. J. Biol. Chem. 275(14):10429-10436(2000)
Wada, I., et al. J. Biol. Chem. 266(29):19599-19610(1991)

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$ 365.00
$ 140.00
Cat# AP13250B
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