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PNPLA1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8N8W4 |
| Other Accession | NP_001139188.1, NP_001139189.2, NP_775947.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57875 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 285848 |
|---|---|
| Other Names | Patatin-like phospholipase domain-containing protein 1, 311-, PNPLA1 |
| Target/Specificity | This PNPLA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PNPLA1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PNPLA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PNPLA1 (HGNC:21246) |
|---|---|
| Function | Omega-hydroxyceramide transacylase involved in the synthesis of omega-O-acylceramides (esterified omega-hydroxyacyl-sphingosine; EOS), which are extremely hydrophobic lipids involved in skin barrier formation (PubMed:27751867, PubMed:28248318). Catalyzes the last step of the synthesis of omega-O-acylceramides by transferring linoleic acid from triglycerides to an omega-hydroxyceramide (PubMed:27751867, PubMed:28248318). Omega-O-acylceramides, are required for the biogenesis of lipid lamellae in the stratum corneum and the formation of the cornified lipid envelope which are essential for the epidermis barrier function (PubMed:22246504, PubMed:27751867, PubMed:28248318). These lipids also play a role in keratinocyte differentiation (By similarity). May also act on omega-hydroxylated ultra-long chain fatty acids (omega-OH ULCFA) and acylglucosylceramides (GlcEOS) (By similarity). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Expressed in the digestive system. Expressed in the epidermis of skin keratinocytes. Strongly expressed in the granular layer. Expressed in the upper epidermis and eccrine sweat glands of the dermis and in the region of keratin filament bundles, which is more pronounced in upper epidermal layers and in the lower cornified layers |
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Provided below are standard protocols that you may find useful for product applications.
Background
Human patatin-like phospholipases, such as PNPLA1, have been implicated in regulation of adipocyte differentiation and have been induced by metabolic stimuli (Wilson et al., 2006 [PubMed 16799181]).
References
Kienesberger, P.C., et al. J. Lipid Res. 50 SUPPL, S63-S68 (2009) :
Johansson, L.E., et al. PLoS ONE 4 (4), E5327 (2009) :
De Chaudhuri, S., et al. Environ. Health Perspect. 116(4):501-505(2008)
Wilson, P.A., et al. J. Lipid Res. 47(9):1940-1949(2006)
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